Description

Collect multiple metrics from a BAM file

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

bam:file

SAM/BAM/CRAM file

*.{sam,bam,cram}

bai:file

Optional SAM/BAM/CRAM file index

*.{sai,bai,crai}

meta2:map

Groovy Map containing reference information e.g. [ id:‘genome’]

fasta:file

Genome fasta file

meta3:map

Groovy Map containing reference information e.g. [ id:‘genome’]

fai:file

Index of FASTA file. Only needed when fasta is supplied.

*.fai

Output

name:type
description
pattern

metrics

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_metrics:file

Alignment metrics files generated by picard

*_{metrics}

pdf

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.pdf:file

PDF plots of metrics

*.{pdf}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

picard
MIT

A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.